Role of gamma-delta T cells in host response against Staphylococcus aureus-induced pneumonia
- Ping Cheng†1,
- Tao Liu†1,
- Wei-Ying Zhou2,
- Yuan Zhuang1,
- Liu-sheng Peng1,
- Jin-yu Zhang1,
- Zhi-Nan Yin3,
- Xu-hu Mao1,
- Gang Guo1,
- Yun Shi1Email author and
- Quan-ming Zou1Email author
© Cheng et al.; licensee BioMed Central Ltd. 2012
Received: 19 March 2012
Accepted: 20 June 2012
Published: 9 July 2012
Staphylococcus aureus is the major cause of hospital-acquired and community-acquired pneumonia. Host defense to S.aureus infection is largely mediated by the innate immune system. γδ T cells play an important role in innate immunity to many infectious diseases. However, less is known about the role of these cells during S.aureus-induced pneumonia. In this study, we examined the response and the role of γδ T cells to pulmonary S.aureus infection.
Mice infected with S. aureus intranasally showed rapid γδ T cells accumulation in the lung. Deficiency of γδ T cells led to attenuated bacterial clearance and less tissue damage in lung compared with WT mice. Moreover, TCR-δ−/− mice exhibited impaired neutrophil recruitment and reduced cytokine production at the site of infection. The γδ T cells in response to pulmonary S. aureus infection mainly secreted IL-17 and γδ T cells deficiency reduced IL-17 production, which might regulate the production of neutrophil-inducing cytokine/chemokine in the S. aureus-infected lungs.
Accumulation of γδ T cells in the lungs to S. aureus infection is beneficial for bacteria clearance and also contributes to the tissue damage. These cells were the primary source of IL-17, which might influence the recruitment of neutrophils at the early stage of infection.
Staphylococcus aureus (S.aureus) is a Gram-positive, extracellular bacterium that cause a variety of infections, including pneumonia, septic arthritis, bacteremia, endocarditic and cellulitis. S.aureus-induced pneumonia accounts for 20-50% nosocomial pneumonia and 25.5% of community-acquired pneumonia, which causes especially severe pulmonary infection and is associated with high morbidity and mortality[2, 3]. In recent years, the rapid emergence of Methicillin-resistant S.aureus raises great concern about the cellular and molecular mechanisms of host defense against S.aureus infection.
Innate immune response plays a critical role in the host defense against S.aureus infection. Rapid and massive neutrophil recruitment to the site of infection is essential for host defense against invading pathogenic microbes. In a mouse model of S. aureus pneumonia, depletion of neutrophils resulted in delayed bacteria clearance and decreased survival of infected mice. Following S.aureus challenge, alveolar macrophages and dendritic cells (DC) are also recruited to the site of infection and contribute to bacterial clearance, but might also exacerbate lung inflammation and injury[6, 7]. Thus, early interactions between these innate cells and pathogens are important in determining the outcome of S.aureus-induced pneumonia.
In addition to these conventional innate cells, T cells bearing the γδ TCR (γδ T cells), another subset of innate immune lymphocytes, play an essential role in the mucosal host defense bacteria infection. Previous studies suggest that γδ T cells play an important role in regulating the initial immune response to lung pathogens via influencing the recruitment of neutrophils, DC or macrophages. Although the lung γδ T cells comprise a very small percentage of pulmonary immune cells, they seem to be the first line of immune response against invading microbial pathogens. Depletion of γδ T cells led to impaired host defense to lung infections by Klebsiella pneumonia, Mycobacterium tuberculosis or Streptococcus pneumoniae. It has been reported that γδ T cells also play an important role in cutaneous S. aureus infection. However, the exact roles of these cells played in S.aureus-induced pneumonia have not been well defined.
In the present study, we first observed an accumulation of γδ T cells in lungs infected with S. aureus. To investigate their role during an acute S. aureus pneumonia, we assessed the effects of the deficiency of γδ T cells on the host defense against S.aureus infection. Our results demonstrate that γδ T cell knock out attenuated bacterial clearance and resulted in less severe lung damage. Furthermore, a defect of IL-17 induction and impaired neutrophil recruitment following S. aureus challenge was observed in the absence of γδ T cells. We presumed that the production of IL-17 by γδ T cells had effects on early neutrophil-mediated lung inflammation and acute tissue injury.
γδ T cells were accumulated in the lungs infected with S. aureus
Deficiency of γδ T cells led to attenuated bacterial clearance but less acute severe lung damage
Lung neutrophils infiltration in response to S. aureus pulmonary infection was impaired in TCR-δ−/− mice
IL-17-producing γδ T cells provided early responses to S. aureus challenge
Decreased expression of neutrophil-inducing cytokine/chemokine in infected lungs of TCR-δ−/− mice
In this study, we investigated the role of γδ T cells in the early host response to S. aureus infection. A significant accumulation of γδ T cells at lungs was observed as early as 6 h post-infection. The local expansion of resident γδ T cells and/or recruitment from the peripheral circulation may be responsible for the accumulation of γδ T cells in the lungs following S.aureus infection. In murine lungs, Vγ1+ and Vγ4+ γδ T cells subsets are two major subsets[10, 13]. Generally, Vγ4+ γδ T cells are the dominant population of lung γδ T cells and Vγ1+ T cells are mainly located in lymphoid organs. Both subsets are involved in the immune response to various pulmonary infectious pathogens. For example, in the lungs of BCG-infected mice, substantial Vγ1+ and Vγ4+ γδ T cells accumulate at the site of infection. Concomitant with the expansion of pulmonary Vγ4+ γδ T cells, large amounts of Vγ1+ γδ T cells were recruited to the lungs of infected mice. Also, both subsets of γδ T cells were significantly increased following S. pneumoniae challenge. But conversely, Vγ4+ γδ T cells contributed to the increased size of γδ T cells and ablation of Vγ4+ γδ T cells attenuated neutrophil recruitment and impaired host protection. Similarly, in our present study, both Vγ1+ and Vγ4+ γδ T cells were increased in the lungs. Whereas Vγ4+ γδ T cells were the dominant population of the increased pulmonary γδ T cells at the early phase of S.aureus infection. Compared with lung, no significant increase of γδ T cells in the spleen and draining lymph node of infected mice was observed (data not shown). We presume that accumulated γδ T cells were restricted to the lung at the early phase of S.aureu s infection. Of note, approximately 47% of γδ T cells expressing other Vγ genes were unidentified due to limited availability of antibodies in our lab. These cells may include remaining pulmonary Vγ2+ γδ T cells and/or Vγ6+ γδ T cells, as suggested by previous studies. These subsets have not been well defined in the course of pulmonary infection. To determine whether γδ T-cell subsets have a differential role in the immune response to S.aureus-induced pneumonia will require further studies.
γδ T cells exert different influences on the host response in a variety of infection models. In some experimental animal models of pulmonary infections, γδ T cells were demonstrated to be protective against pathogens infection, such as M. tuberculosis, Klebsiella pneumoniae[8, 11], Nocardia asteroides and Cryptococcus neoformans. In these infections the absence of γδ T cells rendered mice more susceptible to pathogen infection. In contrast, other studies reported that γδ T cells were not necessary to the host defense against microbial infection and even harmful to the host. Following challenge with Candida albicans or Salmonella choleraesuis, γδ T cell knock out mice were resistant to infection and showed an accelerated pathogens clearance. So these data indicate that γδ T cell might exert diverse function in different infection disease depending on the unique of bacterial infection. In our current study, compared with wild-type mice, γδ T cell deficient mice show no significant change in survival. But the absence of these cells led to attenuated bacterial clearance. It appears that accumulated γδ T cells in the lungs might contribute to the host protective responses against S.aureus infection.
Contrastingly, regardless of bacteria load, we found that mutant mice exhibited less severe pulmonary lesions at the early stage of infection. Since neutrophils are responsible for the acute inflammation response to most infection in the lung, we next detected the neutrophils infiltration. The results showed that the infiltration of neutrophils in the lungs was less in TCR δ−/− mice. It is well known that recruited neutrophils provide the first line of defense against many infections by ingesting the foreign invaders and limiting the pathogen survival and dissemination. It is reported that depletion of neutrophils led to a fatal defect in murine pulmonary S.aureus clearance. On the contrary, neutrophils have also been implicated in the pathology of many inflammatory conditions by releasing oxidants and hydrolytic enzymes. Earlier studies reported that respiratory syncytial virus (RSV) could enhance neutrophils infiltration to the lung and the activated neutrophils significantly augmented RSV induced damage. And pulmonary neutrophil infiltration is an insidious killer in polymicrobial sepsis, in patients with an acute bacterial infection, primed neutrophils with increased oxidative product formation contributed to the damage of pulmonary vascular endothelium during bacteremia. Previous studies also reported that neutrophil recruitment during S. aureus USA300-induced acute pneumonia led to serious lung inflammation and injury, which was associated with the overwhelming release of cytotoxic granule contents. Similarly, other reports demonstrated that the accumulated neutrophils at the site of infection may exacerbate the severity of S. aureus wound infection. Our present study also suggested that the reduced infiltration of neutrophils might be responsible for both the increased bacterial dissemination and the less tissue damage in TCR δ−/− mice.
Consistent with the decreased recruitment of neutrophils to S.aureus infection in the TCR δ−/− mice, we also found decreased KC, MIP-2, GM-CSF, TNF-a, IL-6 and IL-17 in lung. These factors are known to regulate the neutrophil infiltration and their reduction might contribute to the less neutrophil infiltration. IL-17 is worthy of note among these factors, since it has been shown to be a crucial regulator of the migration and activation of neutrophils and also secreted by the γδ T cells. γδ T cells are responsible for much of this rapid cytokine production in the earliest stages of an inflammatory response. Some groups reported that IL-17A-producing cells were essential for vaccine efficacy against systemic S. aureus infection[28, 29]. Previous study reported that IL-17 deficient mice displayed impaired host defense against mucoepithelial infection by S. aureus. Similarly, Kudva et al. described the impact of IL-17-mediated immune response against S. aureus pneumonia. Mice deficient in IL-17R showed impaired bacteria clearance from the lung. In this study, we found that the main source of IL-17 in S.aureus-infected lung was γδ T cells and depletion of γδ T cells results in the reduced IL-17 expression in the early phase of infection. These results implied that the impaired neutrophil accumulation in γδ T cell-deficient mice was likely caused, at least in part, by the decreased production of IL-17 at the site of infection. Nevertheless, no direct evidence of the involvement of IL-17-producing γδ T cells was presented. We cannot exclude the possibility that other products of γδ T cells may have effects on the early neutrophil recruitment. The role for IL-17-producing γδ T cells in the S.aureus-induced pneumonia awaits further study.
In conclusion, γδ T cells accumulated in the lungs after S.aureus infection, which was the primary source of IL-17 at the early phase of infection. Its presence is beneficial for bacteria clearance but also contributes the early acute inflammation and tissue injury in lung. The function of γδ T cells in S. aureus-induced pneumonia might be mediated by neutrophil infiltration. Overall, our findings provide insight into the complex innate immune system in early pulmonary S.aureus infection. Further study is required to elucidate the detailed mechanisms by which γδ T cells control the bacteria clearance and facilitate the tissue damage, which might be helpful to find a way to regulate the γδ T cell to benefit bacteria clearance and avoid the tissue damage.
Six- to 8-wk-old female C57BL/6 mice were purchased from the Experimental Animal Center of the Third Military Medicine University. B6.129P2-Tcrdtm1Mom/J (B6 TCR-δ−/−) mice were kindly provided by Dr. Zhinan Yin (College of Life Sciences, Nankai University, Tianjin, China). All mice were housed in specific pathogen-free facilities. All experiments were approved by the Animal Ethical and Experimental Committee of Third Military Medical University.
Bacteria strains and mouse pneumonia model
The mouse model of S. aureus pneumonia was established as previously described. Briefly, a clinical isolate of the S. aureus SA75 strain was cultured in Mueller Hinton Agar (MHA) for 24 hours prior to inoculation into Mueller Hinton broth which was then incubated overnight. Then the overnight culture was diluted 1:100 into fresh Mueller Hinton broth (MHB). Bacteria were grown at 200 rpm at 37°C to an optical density at 660 nm of 0.5. Cells were collected and washed twice with PBS. All mice were anesthetized with isoflurane and inoculated intranasally with 5 × 108 or 5 × 109 CFU of S. aureus SA75. Animals were held upright for 1 min post-inoculation. Mock-infected control mice were inoculated intranasally with sterile PBS. Mice were either sacrificed at pre-determined time points or assessed for survival for 48 h.
Isolation of pulmonary leukocytes
Pulmonary leukocytes were isolated as previously described. Briefly, the whole lungs were removed aseptically and cut into small pieces and incubated in RPMI 1640 (Hyclone) containing 20U/ml collagenase type I and 1 μg/ml DNase I (Sigma-Aldrich)for 1 h at 37°C under continuous rotation. And the lung portions were crushed through 40 µm meshed steel sieves. The total cell pellet was resuspended in 3 ml of 40% (v/v) Percoll (Pharmacia, Uppsala, Sweden) and layered onto 3 ml of 80% (v/v) Percoll. After centrifugation at 600x g for 20 min at room temperature, the cells at the interface were collected and washed in PBS. All isolated cells were enumerated and resuspended in RPMI 1640. Total viable cell counts were determined by trypan blue exclusion.
Bronchoalveolar lavage (BAL)
Bronchoalveolar lavage (BAL) was performed as previously described. Briefly, the chest was opened and the trachea was exposed through a midline incision and cannulated with a sterile 22-gauge Abbocath-T catheter and BAL was performed by instillation of two 0.5-ml aliquots of sterile saline. Approximately 0.8 ml of BAL fluid was retrieved per mouse.
Mice were sacrificed at 24 h and 48 h postinfection and the levels of bacterial load were determined by preparing lung and spleen homogenates in PBS and plating serially dilutions on MHA. After 18 h of incubation at 37°C, colony forming units were calculated by standard plate counting and presented as CFU/organ. BAL was collected and plated 10-fold serial dilutions on MHA. Colonies were determined as CFU/ml.
Lung tissue was fixed with paraformaldehyde and embedded in paraffin, sectioned, and stained with hematoxylin and eosin (H&E). A double-blind histological analysis was performed to examine the sections from each lung of mock-infected and infected mice, and each section was given a score of 0–4 (no abnormality to most severe pneumonia) according to established criteria.
FACS was performed to analyze cell surface marker and intracellular cytokine expression. Briefly, Lung cells were isolated as mentioned above and stimulated for 6 h with PMA (50 ng/ml; Sigma-Aldrich, St. Louis, MO), ionomycin (1 μg/ml; Sigma-Aldrich) and Golgistop (BD Pharmingen, San Diego, CA). The cells were blocked with anti-CD16/32 antibody (clone 2.4 G2; BD Biosciences) on ice for 15 min, stained for 30 min with the following specific Abs: FITC-anti-γδ TCR(clone GL3), PECy5.5-anti-TCRβ (clone H57-597) and PerCP-Cy5.5-anti-CD8 (clone 53–6.7) were purchased from eBioscience(San Diego, CA), FITC-anti-GR-1(clone RB6-8 C5), APC-Cy7-anti-CD3 (clone 17A2), PerCP-Cy5.5-anti-CD4 (clone RM4-5) and PerCP-Cy5.5-anti-CD11b (clone M1/70) were from Biolegend, PE-anti-TCR Vγ1 (clone 2.11) and APC anti- TCR Vγ4 (clone UC3) were from Tianjin Sungene (Tianjin, China). Intracellular cytokine staining was performed after fixation and permeabilization, using Perm/Wash solution (BD Biosciences). The cells were then separately stained intracellularly with PE-Cy7-anti-IFN-γ (XMG1.2) and PE-anti-IL-17A (TC11-18 H10) (BD Biosciences). Samples were acquired on FACSCanto II (BD Biosciences). Data were analyzed with Flowjo software or FACSDiva software (BD Biosciences).
Lung RNA isolation and Real-time PCR
Total lung RNA was isolated using Trizol (Gibco), according to the manufacturer’s instructions. DNA was removed from RNA preparations with DNase I digestion (Invitrogen) for 30 min at 37°C. And samples were reverse-transcribed to cDNA using the PrimeScript® RT Master Mix (Takara) according to the manufacturers’ instructions. Real-time PCR was performed on the iQ5 apparatus (BioRad, Hercules, USA). The cycle number at which the various transcripts were detectable, referred to as the threshold cycle (Ct), was compared with that of β2-M and referred to as ΔCt. The relative gene expression was expressed as fold change calculated by the ΔΔ Ct method. The expression of IL-17A was determined by the TaqMan method using primers and probes previously reported. The mRNA of keratinocyte-derived chemokine (KC), macrophage inflammatory protein 2 (MIP-2), GM-CSF, tumor necrosis factor alpha (TNF-α) and IL-6 were measured by SYBR Green Realtime PCR Master Mix (Toyobo) using the previously described primers. Mouse β2-M served as the normalizer.
All data are expressed as means ± standard errors of the means (SEM). The statistical analysis software used was SPSS Version13.0. Normally distributed data were analyzed using Student’s t-test. Otherwise, the non-parametric Mann–Whitney test was used. Survival studies were analyzed using Kaplan-Meier testing. A P value of < 0.05 was considered to represent a statistically significant difference. The data were from at least three independent experiments.
This work was supported by National Natural Science Foundation of China (81000715) and Chongqing Science and Technology Commission (2008BB5114).
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