Residue analysis of a CTL epitope of SARS-CoV spike protein by IFN-gamma production and bioinformatics prediction

BMC Immunology

Table 1 Predicted MHC-peptide binding

Sequence	ANN	SMM	ARB
STFFSTFKC	38169.9	44479.5	16214.6
TFFSTFKCY	34824.9	468823.1	191908.2
FFSTFKCYG	36550.6	165788.7	202312.4
FSTFKCYGV	37493.9	270563	24611.6
STFKCYGVS	37025.3	36095.8	11060.5
TFKCYGVSA	36950.6	857351.7	1000000
FKCYGVSAT	28781	9145.4	180.1
KCYGVSATK	36702.2	4243.3	476309.3
CYGVSATKL	59.2	84.1	3.7
YGVSATKLN	39136.2	114095.1	1000000
GVSATKLND	39141.7	224004.7	1000000
VSATKLNDL	26880.9	23063.8	59717.3
SATKLNDLC	35465.1	10192.3	206549.5
ATKLNDLCF	39187.4	4083163	1000000
TKLNDLCFS	38468.4	807152.2	357259.1
KLNDLCFSN	37970.1	12958.7	203755.1

The binding affinities of 9 aa peptides in S358–382. (STFFSTFKCYGVSATKLNDLCFSN) to H-2 K^d were predicted by computer algorithms. Numbers in the table are values of IC₅₀ (nM) determined by ANN, SMM, and ARB. IC₅₀ = 50% inhibitory concentration (low IC₅₀ values indicate high affinity binding).

ISSN: 1471-2172