Figure 7

CHIPS with C-terminal truncation and selected corresponding mutants have the same overall structure. CD spectra of C-terminally truncated CHIPS and variants with reduced C5aR blocking activity, carrying one of the amino acid substitutions K95S, Y97K, K100A or S107N, were recorded. All CHIPS variants have a significant absorption minimum at 205 nm and are suggested to have the same overall structure as the corresponding wt protein.