Figure 4

MMP-9, but not other MMPs, is specifically regulated by PAK1. (A) HDFs transduced by lentivrus expressing GFP or PAK1 variant (K299R) were embedded in type-I collagen and treated by cytokines for 3 days. MMP-3 in the conditioned medium was measured by Western blot analysis. (B) After 16 hrs of treatment with cytokines, the mRNA of MMPs and TIMP-1 were measured by real-time RT-PCR, normalized by GAPDH (n = 3).