Figure 8

Activation and stabilization of endogenous PAK1 in response to TNF-α by keratinocytes and dermal fibroblast. (A) Keratinocytes (IKCs) were treated by TNF-α for the indicated periods and PAK1 protein was measured by Western blot analysis. Intact PAK1 and the putative degradation and non-specific bands were indicated. (B) Threonine-423 phosphorylation of PAK1 by the IKCs in response to TNF-α was measured by Western blot analysis. (C) Threonine-212 phosphorylation of PAK1 by the IKCs in response to TNF-α was measured by WB analysis. (D) Distribution of phosphorylated PAK1 in cytosolic and membrane compartments was measured by Western blot analysis. GAPDH was used as an indicator for cytosolic proteins. (E) Threonine-423 phosphorylation of PAK1 by HDFs was measured by Western blot analysis. (F) Thronine-212 phosphorylation by HDFs was measured by WB analysis.