Figure 1
From: High expression of 5-lipoxygenase in normal and malignant mantle zone B lymphocytes
Semiquantitative RT-PCR analysis of enzymes in the leukotriene pathway in subpopulations of B cells from tonsils. Semiquantitative RT-PCR analysis was performed on purified non-fractioned B cells (tonsillary), memory B cells, mantle zone B cells and germinal centre B cells. The figure depicts the expression of 5-LO, FLAP, leukotriene A4 hydrolase (LTA4H) and leukotriene C4 synthase (LTC4S). Highest relative expression of 5-LO, FLAP and LTA4H was found in mantle zone B cells. The gene expression of LTC4S was absent in all the three investigated B cell subpopulations. The values are presented as "Transcripts/GAPDH 103 transcripts". Values represent experiments performed from two independent tonsils with a minimum of duplicate RT-PCR experiment per gene (mean ± SD).