Figure 3

Immunofluorescence analysis of 5-LO expression in subsets of tonsillar B lymphocytes. Tonsils were prepared for immunofluorescence analyses and stained with anti-IgD, anti-CD38, anti-CD138 and DAPI. A) B cells within the mantle zone were stained with anti-IgD (green, A1); anti-5-LO (red, A2) and DAPI (blue, A3). The combined picture (A4 (A1+A2+A3)) depicts high expression of 5-LO in IgD⁺ cells. B) Germinal centre B cells were stained with anti-CD38 (green, B1); anti-5-LO (red, B2) and DAPI (blue, B3). The combined picture (B4 (B1+B2+B3)) shows very weak expression of 5-LO in CD38⁺⁺ cells. C) Plasma cells within the germinal centre were stained with anti-CD38 (green, C1); anti-5-LO (red, C2) and DAPI (blue, C3). The combined picture (C4 (C1+C2+C3)) demonstrates no expression of 5-LO in CD38⁺⁺⁺ cells. D) Plasma cells outside the germinal centre were stained with anti-CD138 (green, D1); anti-5-LO (red, D2) and DAPI (blue, D3). The combined picture (D4 (D1+D2+D3)) depicts no expression of 5-LO in CD138⁺ cells. The figure represents one out of four examined tonsils.