Figure 3

T helper cell differentiation profiles driven by Bp -844- or Bt -UE5-stimulated MoDCs. MoDCs were allowed to mature after priming with PFA-fixed Bp-844 or Bt-UE5 for 24 hr. These matured MoDCs were then co-cultured with allogeneic T cells for approximately 2 weeks as detailed in the Methods section. Afterward, the resting differentiated T cells were restimulated with PMA/ionomycin and their intracellular IL-4 and IFN-γ levels were analyzed by flow cytometry. E. coli LPS, poly I:C and E.coli LPS + PGE2 were used as referencing stimuli for mixed Th1/Th2, Th1 and Th2, respectively. Results (mean and SEM) of the T cell responses from 5 different MoDC donors interacting with a same batch of allogeneic T cells are shown as per cent of responsive positive T cells in A and a scatter plot from one representative donor is shown in B. Circles indicate the predominant Th1 cell population induced by Bp-844 and Bt-UE5.