Influence of compound 48/80 on rat dermal microvascular leakage induced by TM-N49 and histamine (A), and on mast cell numbers in the peritoneum of rats (B). Rats were intra-peritoneally injected with compound 48/80 at a dose of 0.6 mg·kg-1 for 6 h, twice a day for 3 days, and doubled dose in day 4 before TM-N49 (5 μg) or histamine (5 μg) being administrated for 20 min. Skin reaction represented the area of Evan's blue extravasation. Data are displayed as a boxplot, which indicates the median, interquartile range, the largest and smallest values other than outliers (O) (defined as those which are more than 1.5 box lengths from the median) for 6 animals in each group. In (A), * P < 0.05 compared with the corresponding response to untreated animals and in (B), * P < 0.05 compared with the response to untreated animals.