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Table 2 Gene specific primers used in this study

From: Bos taurus genome sequence reveals the assortment of immunoglobulin and surrogate light chain genes in domestic cattle

Primer

 

Sequence

VPREB1

  

VPREB1-f2

C, E

5'-catgtcctgggccctcgt-3'

VPREB1-r1

C, E

5'-gcccagcctccttgtccac-3'

VPREB3

  

VPREB3-fw1

C, E

5'-tgtgtggaggtcccgaag-3'

VPREB3-fw2

C

5'-cgcagaacagcggactcct-3'

VPREB3-rev1

C, E

5'-aggtcaggagtagaagtgg-3'

IGLL1

  

L5-f1b

C

5'-ccagcgcgtctgcccaag-3'

L5-f2c

E

5'-tgctggctgggcgtctgg-3'

L5-r3a

C, E

5'-agaagggacgtaggggaccat-3'

GAPDH

  

QGAPDfw

E

5'-ctgacctgccgcctggag-3'

QGAPDrev

E

5'-aagagtgagtgtcgctgttgaag-3'

  1. The use of each primer is indicated: C (cloning of the full length cDNA), E (RT-PCR expression analysis).
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BMC Immunology

ISSN: 1471-2172

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