Figure 3
An overview of methods of measuring ovine neutrophil respiratory burst function. Ovine neutrophil respiratory burst function was characterised by V max , time to V max , the total amount of O2- released and the reaction duration. V max was calculated from the 5 data-points corresponding to the steepest gradient of the graph as described in Materials and Methods. The time of V max was defined as the middle of these 5 data points, and the time taken to reach V max was calculated as the time interval between agonist addition and the time of V max . The average OD of the 5 data-points immediately preceding agonist addition was calculated, and the difference between this initial OD and the maximum OD reached in the reaction was used to calculate the total amount of O2- generated in the reaction as described in Methods. The reaction duration was calculated as the time interval between agonist addition and the time when the maximum OD was reached.