Figure 3
CXCL12 stimulation primarily activates Gαi2.(A) Jurkat TAg T cells were tranfected with G-specific siRNA (GNAQ1103), Gαi2-specific siRNA (GNAI-2-1050) or control siRNA (GNAQ1103 M3). 48 hours post-transfection cells were pre-treated with Wortmannin for 30 minutes and analyzed for CXCL12 dependent and independent migratory responses. Migration index represents fold migration over basal in control cell wells. (B) Jurkat TAg T cells were transfected with Lck Y505F or empty vector migration was analysed in the absence and presence of CXCL12 (10 ng/ml). Migration index represents fold increase in migration over basal migration in wells with control cells. (C) Cells were subjected to tranfection as in (B) pre-treated with Wortmannin as in (A) and migration with CXCL12 was analysed. Migration index represents fold increase in migration over basal migration in wells with control cells.