Figure 2

Comparison of tonsil B lymphocytes isolated using RosetteSep with added human or sheep RBCs. A. Unpurified tonsil cells, and B lymphocytes isolated using RosetteSep without added RBCs (n = 5), or with sheep RBCs (n = 2), or with human RBCs (n = 9), were analyzed by flow cytometry using anti-CD19 and anti-CD3, as indicated. Percentages of CD19-positive and CD3-positive cells in a representative experiment are shown. B. Reverse transcription PCR was used to further examine the purity of the tonsil B lymphocyte preparations. A PCR product for CD3-γ was amplified from cells purified with either no added RBCs or sheep RBCs (upper panel), but no PCR product was detected in cells purified using added human RBCs (n = 2). CD20 and beta actin were amplified to show that there were equal amounts of cDNA used for amplification.