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Figure 5 | BMC Immunology

Figure 5

From: The expression of the β-defensins hBD-2 and hBD-3 is differentially regulated by NF-κB and MAPK/AP-1 pathways in an in vitro model of Candida esophagitis

Figure 5

NF-κB is involved in the induction of hBD-2 but not hBD-3 expression. OE21 cells were transfected with a control siRNA or a siRNA directed against RelA/p65. A) Total protein extracts were submitted to Western Blotting for the analysis of RelA/p65 using HSP90 for normalization and B) EMSA on nuclear extracts of siRNA transfected OE21 cells treated 9 with 1 × 105 C. albican/ml for 1 h. Representative results from three independent experiments are shown. C) SiRNA transfected OE21 cells were incubated with 1 × 105 C. albican/ml for 24 h and hBD gene expression was assessed by real time RT-PCR. Means ± s.d. of three independent experiments performed in triplicate are shown, * indicates p-value <0,05.

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BMC Immunology

ISSN: 1471-2172

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