Figure 5
From: Conserved cryptic recombination signals in Vκ gene segments are cleaved in small pre-B cells
Vκ cRS cleavage is detected only in pre–B cells from RAG2:GFP knock-in mice. LM-PCR was conducted on pro-, pre-, and immature B cells from RAG2:GFP knock-in mice [25]. These sorted cells were shown in [25] to have the appropriate lineage and developmental restrictions of Tdt and RAG1 expression, and of JH and Jκ SE. Samples from these same sorted cells were used to amplify SE in Vκ 6 gene segments. Cleavage at Vκ 6-embedded cRS was detected only in pre-B cells. CD14 amplification demonstrates the equivalence of genomic template.