Figure 4
From: Identification in milk of a serum amyloid A peptide chemoattractant for B lymphoblasts
Chemoattractant activity of < 10 kDa fraction (A) and of synthetic peptides (B) with MLN lymphocytes enriched in CD3 or depleted in SIg+ B cells (at a concentration of 3.7 μM). A) CD3+ (F+), CD3- (F-) and origin cell (FO) fractions with the < 10 kDa fraction of whey (UF10) and corresponding spontaneous migration (fMLP 10-6 M) and RPMI controls with FO, F+ and F-). B) Chemoattractant activity of 1042 Da (PepA) or 2735 Da (PepB) synthetic peptide with the SIg+ B cell-enriched fraction (F sIg+) and chemoattractant activity of the same 1042 Da (PepA) and 2735 Da (PepB) peptides with the fraction depleted of SIg+ B cells (F sIg-). Corresponding spontaneous migration (fMLP 10-6 M and RPMI controls). Results are the mean number of adherent cells ± SEM per 4 high-power fields (HPF).