Figure 4

HIV Gag-specific CD8⁺ T cell responses. Mice were primed with a DNA vaccine encoding DEC205-targeted HIV p41 Gag (DEC-p41) or control plasmids (Con-p41, mock) with or without CpI:C prior to boosting with an adenoviral vectors encoding HIV GagPol (Ad-Hgp^syn) or GFP (Ad-GFP). One week after the boost, the percentage of CD8⁺ cells also positive for IFN-γ and CD107a (A) or IFN-γ and IL2 (B) after stimulation with an immunodominant HIV Gag peptide were determined. Mean percentages with SEM of two independent experiments with a total of eight mice/group are shown. For reason of clarity, the level of significance in the Bonferroni Multiple Comparison test is only indicated for selected group to group comparisons (* p < 0.05; ** p < 0.01; ***p < 0.001).