Figure 4

Impaired IFN-γ production in DC-T cell cocultures is due to altered T cell, but not DC, function after burn injury. Cells were prepared from mouse spleens 5 days after sham or burn injuries. Naïve T cells, co-cultured with HKPA-stimulated DCs, were stimulated with Concanavalin A and media were harvested 24 hours later. Cells were isolated from 3 mice and cultures were performed in quadruplicate. IFN-γ levels were measured in co-culture supernatants by ELISA. Data show means +/- SD. *significantly different from cultures containing sham T cells, p < 0.05.