Conclusive and Inconclusive Clonotypes. Ex vivo PBMC and IPP-expanded cell lines were fractionated by magnetic bead cell separation into CD56+ and CD56- populations. RNA was extracted and converted to DNA. Vγ2 chains were sequenced and the frequency of clonotypes within each population was calculated. Limit of detection of this assay was >3%. Clonotypes were considered committed to either the CD56+ or CD56- populations if the distribution was greater than 2:1 (Conclusive); the remaining clones were considered Inconclusive as there was no clear bias in their distribution. Black shading identifies clonotypes committed to CD56+ population, while those committed to the CD56- population are shaded in gray.