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Figure 2 | BMC Immunology

Figure 2

From: Control of CD56 expression and tumor cell cytotoxicity in human Vγ2Vδ2 T cells

Figure 2

Conclusive and Inconclusive Clonotypes. Ex vivo PBMC and IPP-expanded cell lines were fractionated by magnetic bead cell separation into CD56+ and CD56- populations. RNA was extracted and converted to DNA. Vγ2 chains were sequenced and the frequency of clonotypes within each population was calculated. Limit of detection of this assay was >3%. Clonotypes were considered committed to either the CD56+ or CD56- populations if the distribution was greater than 2:1 (Conclusive); the remaining clones were considered Inconclusive as there was no clear bias in their distribution. Black shading identifies clonotypes committed to CD56+ population, while those committed to the CD56- population are shaded in gray.

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