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Figure 2 | BMC Immunology

Figure 2

From: Induction of granzyme B expression in T-cell receptor/CD28-stimulated human regulatory T cells is suppressed by inhibitors of the PI3K-mTOR pathway

Figure 2

Granzyme B expression patterns by flow cytometry in enriched Tregs freshly isolated (unstimulated) or Tregs treated with IL-2 (250 IU/mL) or anti-CD3 coated beads (4:1 ratio of beads to cells) or anti-CD3/anti-CD28 coated beads (4:1 ratio of beads to cells) plus IL-2 (250 IU/ml) for 4 days. Granzyme B expression in the FOXP3+ gated Treg population at left (labeled Tr) is shown in histogram form at right (top right). For ease of comparison, the bottom right plot shows the level of staining of CD3/CD28/IL-2 stimulated cells with a granzyme B isotype control antibody. The data is representative of more than three experiments using multiple donors.

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BMC Immunology

ISSN: 1471-2172

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