Figure 9

OK-432 promotes Syk or PI-3 kinase phosphorylation in MOs. MOs, isolated by the monocyte negative isolation kit, were incubated either with piceatannol, Pic. (30 μM) or diluent control (A) or LY294002, LY (50 μM) (B) and either left unstimulated or further stimulated with OK-432 for 15 min. Blots shown are representative of two separate experiments performed with pooled MOs obtained from two different blood donors.