Figure 3

Time course of protein expression of ChaFFs in Nb infected lungs. Western blot analysis of lung lavages harvested at different time points post Nb infection (4 Nb mice per timepoint) suggested that the secretion of ChaFFs RELMα (A) and Ym1 (B) peaked around 5-7 days post infection. Data are expressed as percentage of a positive control sample (pooled lung lavages of three Nb-infected mice). Representative photomicrographs from Nb-infected lung tissue sections stained histologically for Ym1 at the indicated time points, plus an uninfected control lung (C), illustrate peak expression of brown Ym1 staining of epithelial cells and macrophages (E-F).