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Figure 3 | BMC Immunology

Figure 3

From: Combined TLR2 and TLR4 ligation in the context of bacterial or helminth extracts in human monocyte derived dendritic cells: molecular correlates for Th1/Th2 polarization

Figure 3

Distinct mRNA expression levels after stimulation of immature DCs with Th1 and Th2 inducing compounds. A. mRNA expression levels of the different genes compared to LPS (representing a value of 1). Green and red colours represent expression levels higher and lower, respectively, relative to LPS pulsed DCs. Expression was determined by real-time PCR with TAF-1 as housekeeping gene. Stimuli were clustered hierarchically according to expression profiles (top of figure). B and C. Amount of IL-12 p70 and IL-23 present in the supernatant of DCs 48 hours after stimulation, relative to the amount produced in the presence of LPS only (2518 ± 1733 pg/ml IL-12 & 230 ± 195 pg/ml IL-23). ND: not detectable, * p < 0.05, ** p < 0.01 compared to LPS stimulation only. D and E. Association of delta-4 (D) and c-fos (E) mRNA expression levels with T cell polarization for TLR2 activating stimuli. Diamonds represent HKLM or E. coli, circles the helminth derived lipids. Relative expression levels to LPS control condition (set to 1) from at least 3 independent experiments are shown.

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BMC Immunology

ISSN: 1471-2172

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