Gallin; an antimicrobial peptide member of a new avian defensin family, the ovodefensins, has been subject to recent gene duplication

Table 1 Names and sequences of the primer pairs used in the study

Forward primer name	Forward primer sequence	Reverse primer name	Reverse primer sequence	Position of amplified segment chr3:
Genomic
Gallin#1Exon1L	GCTCACCCCCA GACTGAATA	GallinExon1R	CTCTTCAGAGGC ACGGTGTT	109,913,588- 109,914,151
Gallin#1Exon2L	CTCCAAACCAT TGGCTGACT	GallinExon2R	GGCAAAAGGTGA CTCTGAGC	109,912,734- 109,913,390
Gallin#2Exon1&2L	TCCACGTGTTC AGCTCTTTG	Gallin#2Exon1&2R	CTCTGTGCCATTC CCATTG	109,920,204- 109,921,009
Gallin1#3Exon1&2L	CGAAGTCAGTG ATTTTCTTTCG	Gallin#3Exon1&2R	GAAGGACACCAA GGCAATGT	109,923,361- 109,924,111
cDNA
Gallin AllF1	CTCCAGCCTCG CTCACAC	GallinGenomic1R2	TTGAGAGGAGGG GATGACAC
GallinF	AGGCTATGGGC TGGTCCTGAA	GallinR	TCCTCAGCCCTTA TTTCCACT
GallinEXF	AGGCCTGCAGC TGGTCCTGAA	GallinEXR	TCCTCTGCAGTTA TTTCCACT

¹Primer pairs used in re-sequencing of the 3 gallin forms in the genome, for measurement of cDNA and for recombinant expression studies. All positions refer to the May 2006 chicken (Gallus gallus) v2.1 draft genomic sequence assembly where appropriate.

ISSN: 1471-2172