Figure 2

Dose-dependent neutralization of the RNA-NGA of Stx2 by antibodies of high and medium HeLa cell cytotoxicity neutralization groups. The letters 'H' or 'M' within parenthesis after a HuMAb designation represent high or medium HeLa cell cytotoxicity-neutralization group/s of the antibodies, respectively. The superiority of 5C12 over 6D8 and 6B7 to neutralize RNA-NGA of Stx2 was apparent at all doses, especially at the lower doses of 93.7 and 46.8 ng. At the 46.8 ng dose, the quantitative expression of the luciferase band in presence of the 5C12, 6D8 and 6B7 relative to when Stx2 was absent was 0.72, 0.37 and 0.44, respectively. The experiment was repeated once with similar results.