Table 1 Oligonucleotide primers used to amplify the gcCXCR5 gene

F1

ATCTACCTGCTA(G)CAC(T)CTGGC

Cloning for the internal fragment

R1

AGCAGC(G)TGC(G)AGCAGGTCG(T)C(T)T

UPM Long

CTAATACGACTCACTATAGGGCAAGCAGTGGTATCAACGCAGAGT

Race-PCR Universal primers

UPM Short

CTAATACGACTCACTATAGGGC

AAP

GGCCACGCGTCGACTAGTACGGGIIGGGIIGGGIIG

5' RACE Abridged Primer

AUAP

GGCCACGCGTCGACTAGTAC

5-R1

GGTTCCAAAAAGCCA

5'RACE 1^st round PCR

5-R2

CTTGAGTAACTGCGAATGGAAA

5'RACE 2^nd round PCR

5-R3

GAGCAGGTCAGCCAGTGCCAGAT

5'RACE 3^rd round PCR

3-F1

GTTTTCTGCCTGTGCTGGCTACCG

3'RACE 1^st round PCR

3-F2

CCCTGGTAGTGTTGGGTGTTATGCC

3'RACE 2^nd round PCR

3-F3

CCGTTTCCGAAGAGACCTCCTGC

3'RACE 1^st round PCR

3-F4

GCCATTACTACCACAACAAGCAGC

3'RACE 2^nd round PCR

D-F1

TGGACGAGGACACACACTTCTGAG

For intron

D-R1

GCCGTTTTAGCAGGACTGTCAAGAG

β-actinF

CCTTCTTGGGTATGGAGTCTTGAGAGTATTTACGCTCAGGTGGG

Real-time quantitative PCR control

β-actinR

RT-F1

GTGTTGGGTGTTATGCCTGAGATGTATTGGCTGCTTGTTGTG

Real-time quantitative PCR

RT-R1