Figure 2

Cytotoxicity of the D2NK cells. (a) Natural killing activities of NK92, NKL cell lines and in vitro expanded primary D2NK cells. The effector cells were NK92, NKL, D2NK cells, the target cells were K562 cells; the effector: target ratio were 10:1, and 1:1; reaction at 37°C for 4 hours. (b) Natural killing and ADCC of D2NK cells against normal and WNV infected Vero cells. The ADCC group was done with effector cell, target cells, and WNV specific hyper-immune ascetic fluid; the natural killing group was done with effector cells, target cells, but no antibody; the Ab control group was done with effector cell, target cells, and HIV-1 RT specific ascetic fluid as nonspecific antibody and ascetic fluid control; the target cells were Vero cells either uninfected or 48 hours post infection with 100 TCID50 units of WNV; the effector cells were in vitro expanded D2NK cells; the E:T ratio was 10:1; WNV hyper-immune acetic fluid and HIV-1 RT McAb in acetic fluid were used at 1:1000 dilutions for ADCC; All reactions were performed in triplicate. The SD is the standard deviation from 6 tests with NK cells expanded from 6 different donor PBMC.