Table 2 Hamster primers and probes used for duplex real-time RT PCR
Hamster target gene | Primers and probes sequences | Amplicon length (bp) | |
|---|---|---|---|
γ-actin | γactin-For | 5'-ACA GAG AGA AGA TGA CGC AGA TAA TG-3' | 70 bp |
|  | γactin-Rev | 5'-GCC TGA ATG GCC ACG TAC A-3' |  |
|  | γactin-Probe | 5'-VIC - TTG AAA CCT TCA ACA CCC CAG CC-(TAMRA)-3' |  |
IL-10 | IL-10-F | 5'-GGT TGC CAA ACC TTA TCA GAA ATG-3' | 194 bp |
| Â | IL-10-R | 5'-TTC ACC TGT TCC ACA GCC TTG-3' | Â |
| Â | IL-10-P | 5'-(6FAM) TGC AGC GCT GTC ATC GAT TTC TCC C-(TAMRA)-3' | Â |
IL-4 | IL-4-F | 5'-ACA GAA AAA GGG ACA CCA TGC A-3' | 95 bp |
| Â | IL-4-R | 5'-GAA GCC CTG CAG ATG AGG TCT-3' | Â |
| Â | IL-4-P | 5'-(6FAM) AGA CGC CCT TTC AGC AAG GAA GAA CTC C-(TAMRA)-3' | Â |
IFN-γ | IFN-γ-F | 5'-TGT TGC TCT GCC TCA CTC AGG-3' | 130 bp |
|  | IFN-γ-R | 5'-AAG ACG AGG TCC CCT CCA TTC-3' |  |
|  | IFN-γ-P | 5'-(6FAM) TGG CTG CTA CTG CCA GGG CAC ACT C-(TAMRA)-3' |  |
IL-13 | IL-13-F | 5'-AAA TGG CGG GTT CTG TGC-3' | 81 bp |
| Â | IL-13-R | 5'-AAT ATC CTC TGG GTC TTG TAG ATG G-3' | Â |
| Â | IL-13-P | 5'-(6FAM)-TGG ATT CCC TGA CCA ACA TCT CTA GTT GC (TAMRA)-3' | Â |
IL-21 | IL-21-F | 5'-GGA CAG TGG CCC ATA AAA CAA G-3' | 80 bp |
| Â | IL-21-R | 5'-TTC AAC ACT GTC TAT AAG ATG ACG AAG TC-3' | Â |
| Â | IL-21-P | 5'-(6FAM)-CAA GGG CCA GAT CGC CTC CTG ATT-(TAMRA)-3' | Â |
TGF-β1 | TGFβ1-F | 5'-GGC TAC CAC GCC AAC TTC TG-3' | 81 bp |
|  | TGFβ1-R | 5'-GAG GGC AAG GAC CTT ACT GTA CTG-3' |  |
|  | TGFβ1-P | 5'-(6FAM)-CCC TGT CCC TAC ATT TGG AGC CTG GA-(TAMRA)-3' |  |
CCL17 | CCL17-F | 5'-GTG CTG CCT GGA GAT CTT CA-3' | 89 bp |
| Â | CCL17-R | 5'-TGG CAT CCC TGG GAC ACT-3'5'-TGG CAT CCC TGG GAC ACT-3' | Â |
| Â | CCL17-P | 5'-(6FAM)-CCA TTC CCA TCA GGA AGC TGG TGA TG-(TAMRA)-3' | Â |
CCL22 | CCL22-F | 5'-TGG TGC CAA CGT GGA AGA C-3' | 82 bp |
| Â | CCL22-R | 5'-GAA GAA CTC CTT CAC TAC GCG C-3' | Â |
| Â | CCL22-P | 5'-(6FAM)-CTG CTG CCA GGA CTA CAT CCG TCA GC-(TAMRA)-3' | Â |
CCR4 | CCR4-F | 5'-GCT TGG TCA CGT GGT CAG TG-3' | 80 bp |
| Â | CCR4-R | 5'-GTG GTT GCG CTC CGT GTA G-3' | Â |
| Â | CCR4-P | 5'-(6FAM)-TCC CTC CCA GGC CTC TTG TTC AGC-(TAMRA)-3' | Â |
FOXP3 | FOXP3-F | 5'-GGT CTT CGA GGA GCC AGA AGA-3' | 72 pb |
| Â | FOXP3-R | 5'- GCC TTG CCC TTC TCA TCC A-3' | Â |
| Â | FOXP3-P | 5'-(6FAM)-TTT CTC AAG CAC TGC CAA GCA GAT CAC C-(TAMRA)-3' | Â |
TNF-α | TNF-α-F | 5'-TGA GCC ATC GTG CCA ATG-3' | 79 bp |
|  | TNF-α-R | 5'-AGC CCG TCT GCT GGT ATC AC-3' |  |
|  | TNF-α-P | 5'-(6FAM)-CGG CAT GTC TCT CAA AGA CAA CCA G-(TAMRA)-3' |  |
IL-12p40 | IL-12p40-F | 5'-AAT GCG AGG CAG CAA ATT ACT C-3' | 88 bp |
| Â | IL-12p40-R | 5'-CTG CTC TTG ACG TTG AAC TTC AAG-3' | Â |
| Â | IL-12p40-P | 5'-(6FAM)-CCT GCT GGT GGC TGA CTG CAA TCA-(TAMRA)-3' | Â |