Skip to main content

Table 2 Hamster primers and probes used for duplex real-time RT PCR

From: Duplex real-time reverse transcriptase PCR to determine cytokine mRNA expression in a hamster model of New World cutaneous leishmaniasis

Hamster target gene

Primers and probes sequences

Amplicon length (bp)

γ-actin

γactin-For

5'-ACA GAG AGA AGA TGA CGC AGA TAA TG-3'

70 bp

 

γactin-Rev

5'-GCC TGA ATG GCC ACG TAC A-3'

 
 

γactin-Probe

5'-VIC - TTG AAA CCT TCA ACA CCC CAG CC-(TAMRA)-3'

 

IL-10

IL-10-F

5'-GGT TGC CAA ACC TTA TCA GAA ATG-3'

194 bp

 

IL-10-R

5'-TTC ACC TGT TCC ACA GCC TTG-3'

 
 

IL-10-P

5'-(6FAM) TGC AGC GCT GTC ATC GAT TTC TCC C-(TAMRA)-3'

 

IL-4

IL-4-F

5'-ACA GAA AAA GGG ACA CCA TGC A-3'

95 bp

 

IL-4-R

5'-GAA GCC CTG CAG ATG AGG TCT-3'

 
 

IL-4-P

5'-(6FAM) AGA CGC CCT TTC AGC AAG GAA GAA CTC C-(TAMRA)-3'

 

IFN-γ

IFN-γ-F

5'-TGT TGC TCT GCC TCA CTC AGG-3'

130 bp

 

IFN-γ-R

5'-AAG ACG AGG TCC CCT CCA TTC-3'

 
 

IFN-γ-P

5'-(6FAM) TGG CTG CTA CTG CCA GGG CAC ACT C-(TAMRA)-3'

 

IL-13

IL-13-F

5'-AAA TGG CGG GTT CTG TGC-3'

81 bp

 

IL-13-R

5'-AAT ATC CTC TGG GTC TTG TAG ATG G-3'

 
 

IL-13-P

5'-(6FAM)-TGG ATT CCC TGA CCA ACA TCT CTA GTT GC (TAMRA)-3'

 

IL-21

IL-21-F

5'-GGA CAG TGG CCC ATA AAA CAA G-3'

80 bp

 

IL-21-R

5'-TTC AAC ACT GTC TAT AAG ATG ACG AAG TC-3'

 
 

IL-21-P

5'-(6FAM)-CAA GGG CCA GAT CGC CTC CTG ATT-(TAMRA)-3'

 

TGF-β1

TGFβ1-F

5'-GGC TAC CAC GCC AAC TTC TG-3'

81 bp

 

TGFβ1-R

5'-GAG GGC AAG GAC CTT ACT GTA CTG-3'

 
 

TGFβ1-P

5'-(6FAM)-CCC TGT CCC TAC ATT TGG AGC CTG GA-(TAMRA)-3'

 

CCL17

CCL17-F

5'-GTG CTG CCT GGA GAT CTT CA-3'

89 bp

 

CCL17-R

5'-TGG CAT CCC TGG GAC ACT-3'5'-TGG CAT CCC TGG GAC ACT-3'

 
 

CCL17-P

5'-(6FAM)-CCA TTC CCA TCA GGA AGC TGG TGA TG-(TAMRA)-3'

 

CCL22

CCL22-F

5'-TGG TGC CAA CGT GGA AGA C-3'

82 bp

 

CCL22-R

5'-GAA GAA CTC CTT CAC TAC GCG C-3'

 
 

CCL22-P

5'-(6FAM)-CTG CTG CCA GGA CTA CAT CCG TCA GC-(TAMRA)-3'

 

CCR4

CCR4-F

5'-GCT TGG TCA CGT GGT CAG TG-3'

80 bp

 

CCR4-R

5'-GTG GTT GCG CTC CGT GTA G-3'

 
 

CCR4-P

5'-(6FAM)-TCC CTC CCA GGC CTC TTG TTC AGC-(TAMRA)-3'

 

FOXP3

FOXP3-F

5'-GGT CTT CGA GGA GCC AGA AGA-3'

72 pb

 

FOXP3-R

5'- GCC TTG CCC TTC TCA TCC A-3'

 
 

FOXP3-P

5'-(6FAM)-TTT CTC AAG CAC TGC CAA GCA GAT CAC C-(TAMRA)-3'

 

TNF-α

TNF-α-F

5'-TGA GCC ATC GTG CCA ATG-3'

79 bp

 

TNF-α-R

5'-AGC CCG TCT GCT GGT ATC AC-3'

 
 

TNF-α-P

5'-(6FAM)-CGG CAT GTC TCT CAA AGA CAA CCA G-(TAMRA)-3'

 

IL-12p40

IL-12p40-F

5'-AAT GCG AGG CAG CAA ATT ACT C-3'

88 bp

 

IL-12p40-R

5'-CTG CTC TTG ACG TTG AAC TTC AAG-3'

 
 

IL-12p40-P

5'-(6FAM)-CCT GCT GGT GGC TGA CTG CAA TCA-(TAMRA)-3'