Skip to main content

Table 2 Hamster primers and probes used for duplex real-time RT PCR

From: Duplex real-time reverse transcriptase PCR to determine cytokine mRNA expression in a hamster model of New World cutaneous leishmaniasis

Hamster target gene Primers and probes sequences Amplicon length (bp)
γ-actin γactin-For 5'-ACA GAG AGA AGA TGA CGC AGA TAA TG-3' 70 bp
  γactin-Rev 5'-GCC TGA ATG GCC ACG TAC A-3'  
  γactin-Probe 5'-VIC - TTG AAA CCT TCA ACA CCC CAG CC-(TAMRA)-3'  
IL-10 IL-10-F 5'-GGT TGC CAA ACC TTA TCA GAA ATG-3' 194 bp
  IL-10-R 5'-TTC ACC TGT TCC ACA GCC TTG-3'  
  IL-10-P 5'-(6FAM) TGC AGC GCT GTC ATC GAT TTC TCC C-(TAMRA)-3'  
IL-4 IL-4-F 5'-ACA GAA AAA GGG ACA CCA TGC A-3' 95 bp
  IL-4-R 5'-GAA GCC CTG CAG ATG AGG TCT-3'  
  IL-4-P 5'-(6FAM) AGA CGC CCT TTC AGC AAG GAA GAA CTC C-(TAMRA)-3'  
IFN-γ IFN-γ-F 5'-TGT TGC TCT GCC TCA CTC AGG-3' 130 bp
  IFN-γ-R 5'-AAG ACG AGG TCC CCT CCA TTC-3'  
  IFN-γ-P 5'-(6FAM) TGG CTG CTA CTG CCA GGG CAC ACT C-(TAMRA)-3'  
IL-13 IL-13-F 5'-AAA TGG CGG GTT CTG TGC-3' 81 bp
  IL-13-R 5'-AAT ATC CTC TGG GTC TTG TAG ATG G-3'  
  IL-13-P 5'-(6FAM)-TGG ATT CCC TGA CCA ACA TCT CTA GTT GC (TAMRA)-3'  
IL-21 IL-21-F 5'-GGA CAG TGG CCC ATA AAA CAA G-3' 80 bp
  IL-21-R 5'-TTC AAC ACT GTC TAT AAG ATG ACG AAG TC-3'  
  IL-21-P 5'-(6FAM)-CAA GGG CCA GAT CGC CTC CTG ATT-(TAMRA)-3'  
TGF-β1 TGFβ1-F 5'-GGC TAC CAC GCC AAC TTC TG-3' 81 bp
  TGFβ1-R 5'-GAG GGC AAG GAC CTT ACT GTA CTG-3'  
  TGFβ1-P 5'-(6FAM)-CCC TGT CCC TAC ATT TGG AGC CTG GA-(TAMRA)-3'  
CCL17 CCL17-F 5'-GTG CTG CCT GGA GAT CTT CA-3' 89 bp
  CCL17-R 5'-TGG CAT CCC TGG GAC ACT-3'5'-TGG CAT CCC TGG GAC ACT-3'  
  CCL17-P 5'-(6FAM)-CCA TTC CCA TCA GGA AGC TGG TGA TG-(TAMRA)-3'  
CCL22 CCL22-F 5'-TGG TGC CAA CGT GGA AGA C-3' 82 bp
  CCL22-R 5'-GAA GAA CTC CTT CAC TAC GCG C-3'  
  CCL22-P 5'-(6FAM)-CTG CTG CCA GGA CTA CAT CCG TCA GC-(TAMRA)-3'  
CCR4 CCR4-F 5'-GCT TGG TCA CGT GGT CAG TG-3' 80 bp
  CCR4-R 5'-GTG GTT GCG CTC CGT GTA G-3'  
  CCR4-P 5'-(6FAM)-TCC CTC CCA GGC CTC TTG TTC AGC-(TAMRA)-3'  
FOXP3 FOXP3-F 5'-GGT CTT CGA GGA GCC AGA AGA-3' 72 pb
  FOXP3-R 5'- GCC TTG CCC TTC TCA TCC A-3'  
  FOXP3-P 5'-(6FAM)-TTT CTC AAG CAC TGC CAA GCA GAT CAC C-(TAMRA)-3'  
TNF-α TNF-α-F 5'-TGA GCC ATC GTG CCA ATG-3' 79 bp
  TNF-α-R 5'-AGC CCG TCT GCT GGT ATC AC-3'  
  TNF-α-P 5'-(6FAM)-CGG CAT GTC TCT CAA AGA CAA CCA G-(TAMRA)-3'  
IL-12p40 IL-12p40-F 5'-AAT GCG AGG CAG CAA ATT ACT C-3' 88 bp
  IL-12p40-R 5'-CTG CTC TTG ACG TTG AAC TTC AAG-3'  
  IL-12p40-P 5'-(6FAM)-CCT GCT GGT GGC TGA CTG CAA TCA-(TAMRA)-3'