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Table 4 Real-time RT PCR Standard curves and validation experiments

From: Duplex real-time reverse transcriptase PCR to determine cytokine mRNA expression in a hamster model of New World cutaneous leishmaniasis

Hamster Duplex real-time RT PCR Slopes of the standard curves Correlation coefficient(r2) Efficiencies Validation experiment
(Absolute value)
  Target gene γ-actin Target gene γ-actin Target gene γ-actin  
IL-10 -3.4094 -3.4745 0.95 0.99 1.96 1.94 0.065
IL-4 -3.0506 -31576 0.98 0.98 2.12 2.07 0.107
IFN-γ -3.2861 -3.3459 0.93 0.93 2.01 1.99 0.059
IL-13 -3.6531 -3.6448 0.97 0.99 1.87 1.88 0.008
IL-21 -3.2268 -3.1831 0.98 0.98 2.04 2.06 0.043
TGF-β1 -3.4493 -3.3399 0.98 0.98 1.94 1.99 0.109
CCL17 -3.1135 -3.1023 0.99 0.98 2.09 2.01 0.081
CCL22 -3.2190 -3.2269 0.99 0.98 2.04 2.04 0.008
CCR4 -3.2155 -3.2528 0.99 0.98 2.04 2.02 0.037
FoxP3 -3.2598 -3.1920 0.98 0.99 2.02 2.05 0.077
TNF-α -3.3286 -3.4026 0.99 0.99 1.99 1.96 0.074
IL-12p40 -3.4180 -3.4917 0.94 0.99 1.96 1.93 0.073
  1. Standard curve slops, correlation coefficient (r2), efficiencies and validation experiment results for hamster Duplex real-time RT PCR assays. The efficiency estimation was calculated by E = 10[-1/slope].