Table 4 Real-time RT PCR Standard curves and validation experiments
Hamster Duplex real-time RT PCR | Slopes of the standard curves | Correlation coefficient(r2) | Efficiencies | Validation experiment (Absolute value) | |||
|---|---|---|---|---|---|---|---|
|  | Target gene | γ-actin | Target gene | γ-actin | Target gene | γ-actin |  |
IL-10 | -3.4094 | -3.4745 | 0.95 | 0.99 | 1.96 | 1.94 | 0.065 |
IL-4 | -3.0506 | -31576 | 0.98 | 0.98 | 2.12 | 2.07 | 0.107 |
IFN-γ | -3.2861 | -3.3459 | 0.93 | 0.93 | 2.01 | 1.99 | 0.059 |
IL-13 | -3.6531 | -3.6448 | 0.97 | 0.99 | 1.87 | 1.88 | 0.008 |
IL-21 | -3.2268 | -3.1831 | 0.98 | 0.98 | 2.04 | 2.06 | 0.043 |
TGF-β1 | -3.4493 | -3.3399 | 0.98 | 0.98 | 1.94 | 1.99 | 0.109 |
CCL17 | -3.1135 | -3.1023 | 0.99 | 0.98 | 2.09 | 2.01 | 0.081 |
CCL22 | -3.2190 | -3.2269 | 0.99 | 0.98 | 2.04 | 2.04 | 0.008 |
CCR4 | -3.2155 | -3.2528 | 0.99 | 0.98 | 2.04 | 2.02 | 0.037 |
FoxP3 | -3.2598 | -3.1920 | 0.98 | 0.99 | 2.02 | 2.05 | 0.077 |
TNF-α | -3.3286 | -3.4026 | 0.99 | 0.99 | 1.99 | 1.96 | 0.074 |
IL-12p40 | -3.4180 | -3.4917 | 0.94 | 0.99 | 1.96 | 1.93 | 0.073 |