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Figure 1 | BMC Immunology

Figure 1

From: The antigen presentation function of bone marrow-derived mast cells is spatiotemporally restricted to a subset expressing high levels of cell surface FcεRI and MHC II

Figure 1

Characterization of three-week old BMMC cultures. Histamine staining: slides treated with rabbit IgG (Panel a, d) vs rabbit anti-histamine (Panel b, e). Toluidine blue staining: 5 × 104 BMMC were prepared onto a cytospin slide, examined respectively with 20× (Panel c) and 100× objectives (Panel f). Surface FcεRI and c-kit expression: BMMC were stained with PE-anti-c-kit and FITC-anti-FcεRI analyzed by flow cytometry. Numerically, 98.7% c-kit + cells are mast cells, including c-kit +, FcεRIhi cells (quadrant 3, 90.84%), and c-kit +, FcεRIlo cells (quadrant 2, 7.85%) (g). The procedures were described in Materials and methods.

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BMC Immunology

ISSN: 1471-2172

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