Table 2 Immunogold (E.M.) Antibody Information.
Primary Antibody | Type, Species, IgG-type (Clone) | Dilution | Source | Secondary antibody | Dilution | Source |
|---|---|---|---|---|---|---|
von Willebrand Factor | Polyclonal, Rabbit anti-Human IgG | 1:200 | Dako, A 0082 | Goat anti-Rabbit | 1:100 | BBI International, EM.GAR20 |
Collagen I | Polyclonal, Rabbit anti-Human IgG | 1:100 | Biogenesis, 2150-0020 | Goat anti-Rabbit | 1:200 | BBI International, EM.GAR20 |
Collagen III | Polyclonal, Rabbit anti-Human IgG | 1:200 | Biogenesis, 2150-0100 | Goat anti-Rabbit | 1:100 | BBI International, EM.GAR20 |
Laminin | Polyclonal, Rabbit anti-Mouse IgG | 1:200 | Cappel, 10765 | Goat anti-Rabbit | 1:50 | BBI International, EM.GAR20 |
Fibronectin | Monoclonal, Mouse anti-Human IgG1 (Clone 568) | 1:200 | Novo Castra, NCL-FIB | Goat anti-Mouse | 1:100 | BBI International, EM.GAF20 |
Collagen IV | Monoclonal, Mouse anti-Human IgG1 (Clone CIV 22(1)) | 1:100 | Dako, M0785 | Goat anti-Mouse | 1:50 | BBI International, EM.GAF20 |
Tenascin-C | Monoclonal, Mouse anti-Human IgG1 (Clone TN2) | 1:50 | Dako, M0636 | Goat anti-Mouse | 1:100 | BBI International, EM.GAF20 |