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Figure 2 | BMC Immunology

Figure 2

From: Recombinant human complement component C2 produced in a human cell line restores the classical complement pathway activity in-vitro: an alternative treatment for C2 deficiency diseases

Figure 2

Analysis of rhC2 glycosylation by SEC-MALS. rhC2 was eluted from a Tosoh Super SW3000 size exclusion column and monitored by normalized differential refractive index (─) and absorbance at 280 nm versus time. Molar mass was analyzed by multi angle light scattering following the total mass by differential refractive index (□), and protein mass by absorbance at 280 nm (Ο). The molar mass contributed by glycosylation is plotted as the difference between the total mass and the mass of the protein (Δ). The average molar mass determined for the total is 99.8 kDa, the protein 84 kDa, and the carbohydrate 15.8 kDa.

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BMC Immunology

ISSN: 1471-2172

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