Figure 5

The cytotoxicitiy of MNCs (AM ± SD) isolated from bone marrow (A), peritoneal lavage (B) and spleen (C). Bone marrow, peritoneal lavages and spleens from pre-vaccinated mice were collected on day 0 and subsequently MNCs were isolated. Half of the MNCs from each experimental group were re-exposed to the antigen - irradiated B16F1 tumor cells and another half was grown without any additional stimulation for 5 days. The cytotoxicity of MNCs was determined as described in Materials and methods and was normalized against the control (mock) treated group. 1×: vaccine + two additional injections of CpG ODNs applied once 90, 60 or 30 days before tumor inoculation; 2×: vaccine + two additional injections of CpG ODNs applied twice 90 and 75 days before tumor inoculation, 60 and 45 days before tumor inoculation or 30 and 15 days before tumor inoculation; 3×: vaccine + two additional injections of CpG ODNs applied three times 90, 75 and 60 days before tumor inoculation, 60, 45 and 30 days before tumor inoculation or 30, 15 and 7 days before tumor inoculation.