Figure 4
Effect of human plasma apoA-I, apoB, recombinant apoE2, apoE3, and LBP on TNF-α and IL-8 productions by primary human differentiated macrophages in response to Mip. Human monocytes/macrophages (5 × 105 cells/0.5 ml per well) were differentiated in a 6-day culture in the presence of 2 ng/ml M-CSF. Recombinant Mip at 1 μg/ml was exposed for 30 min to apolipoproteins or LBP before addition to macrophages. After 4 h stimulation, supernatants were collected and their content in TNF-α and IL-8 were analyzed by ELISA. Each value represents the mean ± SD of triplicates from two experiments. The results are expressed as percent of TNF-α or IL-8 release in the absence of serum. ***: p < 0.0001; **: p < 0.005, *: p < 0.05 determined by comparison with medium alone using Student's t test.