Figure 6
Phenotype of adoptively transferred cells after infusion into TBH mice . Mice with established 4T1 flank tumors received CYP (100 mg/kg i.p.) on day 9 after tumor inoculation, followed by AIT with 50 × 106 B/I activated CFSE-stained lymphocytes on day 10. At days 3, 6 and 10 after AIT, the tDLN were harvested, stained for expression of cell surface markers, and analyzed by flow cytometry. Results shown are gated for CFSE+ cells. A) Dual color analysis of CFSE and CD8 or CD4 or DX5 on Day 3. B) Dual color analysis of CFSE and CD44, CD62L or CD69 on days 3, 6 and 10 after AIT.