Figure 1
B-cell lymphoma cell lines show reduced sensitivity to growth inhibition by TGF-β. B-cell lymphoma cell lines and primary B cells were treated with or without TGF-β (A) and B-cell lymphoma cell lines were treated with or without activin A (B) and [3H]-thymidine incorporation was determined after 72 h. [3H]-thymidine was added for the last 4 or 16 h, for cell lines and primary cells, respectively. Depicted is relative [3H]-thymidine incorporation in percentage, compared to controls of each cell type (mean ± SEM, n = 6 (A), n = 3 - 4 (B)). Treated vs. control groups (A) were subjected to Wilcoxon non-parametric test. TGF-β-treated cells significantly different from the control group are indicated by asterisk (*), p < 0.05. (C) SUDHL-4 and BL-41 cells were labelled with 5 μM CFSE for 10 min and grown for three days with and without TGF-β. CFSE staining intensity was measured by flow cytometry. The data is representative of three similar experiments.