Figure 6

The expression of transgenes-encoded Coccidioides -Ag2/PRA and TK antigens by genetically-transfected primary DCs. The cell-lysates were prepared after 24 h of transfection, and dot-immunoblotting was performed with Ag2/PRA and TK-specific antibodies. Left Panel: An Ag2/PRA dot-immunoblot of 5 μg cell-lysate protein from nontransfected (A), transfected primary DCs (B), and 0.2 μg recombinant Ag2/PRA protein (C). Right panel: A TK Dot-immunoblot of 1 μg cell-lysate protein from nontransfected (A) and transfected DCs (B). Results are from one experiment representative of more than three experiments.