Figure 8

In vitro T cell activation by Coccidioides -Ag2/PRA-cDNA transfected DCs. Panels (A) to (E) are BALB/c mice derived primary DCs, and panels (F) to (I) are JAWS II DCs. The vector-plasmid DNA-transfected DCs (panels B and G) and Coccidioides-Ag2/PRA-cDNA transfected DCs (panels C and H) were co-cultured with autologous splenic lymphocytes (1DC: 16 lymphocytes) for 24 h. The co-cultured cells were stained with CD4, CD8, CD25 and CD69-specific antibodies. Panels (A) and (F) are dot plots for CD4+ and CD8+ cells. Representative dot-plots of splenic lymphocytes (without DCs, as negative control) are shown in panels (D) and (I). Positive control included concavalin-treated splenic lymphocytes (panel E). The values in each quadrant represent the percent number of CD4+ and CD8+ cells. Results are from one experiment representative of three experiments performed in duplicate.