From: Subolesin expression in response to pathogen infection in ticks
Pathogen (isolate/strain) | Inoculum | CF tickmeal | Subolesin expression silencing (%)a | Tick infection ratio (Subolesin/Rs86)b |
---|---|---|---|---|
A. marginale (Oklahoma, OK [33]) | 4.3% infected erythrocytes | Blood from splenectomized calves experimentally infected with isolate stabilate | 89 ± 17* | 0.85 ± 0.09* |
A. marginale (Okeechobee, FL [33]) | 3.3% infected erythrocytes | Blood from splenectomized calves experimentally infected with isolate stabilate | 55 ± 32* | 0.83 ± 0.10* |
A. marginale (Bison) [33] | 7.4% infected erythrocytes | Blood from splenectomized calves experimentally infected with isolate stabilate | 86 ± 17* | 0.95 ± 0.10* |
A. phagocytophilum (NY18) [34] | 50% infected cells | ISE6 cultured tick cells in L15B with 10% FBS | 92 ± 14* | 0.91 ± 0.09* |
F. tularensis (Live Vaccine Strain LVS; ATCC 29684) | 107 CFU/ml | DMEM with 10% FBS | 99 ± 2* | 1.74 ± 0.86* |
E. canis (Ebony) | 2% infected cells | DH82 cultured dog cells in DMEM with 10% FBS | 94 ± 11* | 0.89 ± 0.16* |
E. coli (JM109; Promega) | 107 CFU/ml | DMEM with 10% FBS | 97 ± 3* | 0.92 ± 0.07* |
B. subtilis (culture 125-1 kindly supplied by H. Evers) | 107 CFU/ml | DMEM with 10% FBS | 71 ± 21* | 0.65 ± 0.58 |
P. pastoris (X33; Invitrogen) | 106 CFU/ml | YPD | 80 ± 16* | 0.60 ± 0.31 |