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Figure 3 | BMC Immunology

Figure 3

From: Differential utilization of NF-kappaB RELA and RELB in response to extracellular versus intracellular polyIC stimulation in HT1080 cells

Figure 3

In-polyIC treatment induces effective antiviral protection. HT1080 cells were challenged with virus after various pre-treatments including extracellular and intracellular polyIC for 7 hours. IFNA2A treatment was a positive control to show that biologically significant interferon pre-treatment protects cells against virus. Fu-gene (transfection reagent) and 0.4 μg/ml of ex-polyIC were negative controls to show neither of them alone elicits anti-viral protection. Cell viability was measured with crystal violet staining that shows live cells in purple. A. HT1080 cells were challenged with a serial dilution (from MOI 1 to 0.001) of encephalomyocarditis virus (EMCV). B. Summary of Figure 3A. Amount of input EMCV virus (MOI) that causes 50% (or greater) cell survival across different polyIC treatments. C. HT1080 cells were challenged with a serial dilution (from MOI 1 to 0.001) of vesicular stomatitis virus (VSV). D. Summary of Figure 3C. Amount of input VSV virus (MOI) that causes 50% (or greater) cell survival across different polyIC treatments.

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BMC Immunology

ISSN: 1471-2172

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