Figure 1
From: Human CD8 T cells generated in vitro from hematopoietic stem cells are functionally mature
Characterization of CD8+ T cells generated in vitro. Flow cytometric analysis for the expression of CD8 and CD4 from human UCB-derived HSCs cultured on OP9-DL1 cells for 65 days. CD8+ CD4- single positive (SP) cells were gated, as indicated, and analyzed for the expression of CD27 and CD3, with CD1a expression shown for cells gated as CD3+ CD27- or CD27+ (shaded and clear histograms, respectively). Data are representative of over 6 experiments for CD8/CD4 expression and of 3 experiments for CD27/CD1a expression.