Figure 7
Effect of HKLP titration on proliferation of fresh versus cultured Teff cells and cultured Tregs. Cells were stimulated with different concentrations of HKLP in the absence or presence of anti-CD3. After 4 days proliferation was determined by analyses of thymidine incorporation (A) or CFSE dilution, displayed as MFI values (B). Cultured Treg proliferated in response to HKLP in a dose-dependent manner. Freshly isolated Teff also slightly responded to HKLP, while no effects were observed in cultured Teff. Representative results of 3 independent experiments are shown. The error bars represent standard deviations of duplicate measurements.