Figure 3

Spleen and liver DX5⁺NKT cells display a distinct cytokine secretion pattern in Balb/c mice. Isolated DX5⁺NKT cells were stimulated with antibodies against CD3 and CD28 in the presence of IL-2 for 4, 24 and 48 h, respectively. Control cells were cultured simultaneously without stimulation. Values were subtracted as background. FACS analysis revealed again distinct differences between spleen and liver subsets of DX5⁺NKT cells. Results are given as mean + SEM. Experiments were repeated at least three times (* p < 0.05).