Figure 3

Induction of CD25^- iTreg cells by co-immunization depends on epitope affinity. A, CD25^- iTreg (CD4⁺CD25^-Foxp3⁺) and nTreg (CD4⁺CD25⁺Foxp3⁺) induced in Balb/c mice following co-immunization were counted by flow cytometry and calculated as percentage of Foxp3⁺ cells in CD4⁺CD25^- and CD4⁺CD25⁺ T cells, respectively. Naïve, non-immunized mice. **, p < 0.01, n ≥ 5. Difference was calculated among all groups. Each point represents one mouse. Shown is one of three independent experiments with similar results. B, Induction of highly suppressive CD25^- iTreg cells by co-immunization depends on epitope antigenecity. CFSE labeled DO11.10 CD4⁺ T cells were co-cultured with co-immunization-induced CD25^- iTreg, in the presence of OVA_323-339. Proliferation was determined by flow cytometry as divided KJ1-26⁺ cells versus total KJ1-26⁺ cells. **, p < 0.01, n ≥ 5. Each point represents one mouse. Difference was calculated among all groups. Shown is one of three independent experiments with similar results.