Figure 3
Surface marker measurements of DC phenotype for the effects of Shc blockade on LPS-primed maturation. DCs cultured in medium only were immature, with relative low expression of MHC-II and co-stimulatory markers CD80 and CD86 (no LPS). The administration of LPS induced similar levels of DC maturation, indicated by up-regulation of MHC-II, and CD86, in the presence of transfected Shc siRNA (black) or NEG. siRNA (white) (B and C). However, the co-inhibitory marker CD80 was significantly enhanced when Shc was silenced in LPS-treated DCs (compared to NEG. siRNA) (A). The lower panel of 3A shows histogram overlay of CD80 expression in control (black), LPS-exposed with NEG. siRNA (gray) and LPS-exposed with Shc siRNA (light gray) transfection measured by flow cytometry. Each column represents the mean fluorescence intensity (MFI) ± SE of the three independent experiments (n = 3; **, P < 0.01; *, P < 0.05).