Figure 13

Expression of angiogenic molecules from 3-week mDC cultures. DCs were recovered from different cultures after 3 weeks in EGM (A) or RPMI (B), RNA was extracted and reverse-transcribed. Then, quantitative real-time PCR was performed to analyze several angiogenic molecules in these cells. (A) As determined by ANOVA analysis followed by Dunnett multiple comparison test using pre-culture mDCs as a control, expression of VEGF, HGF, MMP2 and MMP9 was significantly different under some conditions (* p < 0.05). (B) MMP-2 expression levels in the RPMI cultured-group showed statistically significant differences respect to pre-culture mDCs at p < 0.05 as determined by ANOVA analysis. For A and B, samples were run in duplicate in each experiment and further analyzed in duplicate by quantitative real-time PCR. An experiment representative of 2 independent experiments is shown.