Figure 16

Immunological properties of 3-week mDC cultures in EGM. IL-1α (A) and IL-6 (B) were detected by ELISA on reseeded cultures after 48 h stimulation with TNFα and LPS. (A) ANOVA analysis: p < 0.0001. Tukey-Kramer Multiple Comparisons post-Test: Fibronectin vs. collagen, Matrigel, poly-d-lysine, polystyrene and preculture: p < 0.001; vs. gelatin: NS. Collagen vs. gelatin, Matrigel, poly-d-lysine, polystyrene, and preculture: p < 0.001. Gelatin vs. Matrigel, poly-d-lysine, polystyrene, and preculture: p < 0.001. Matrigel vs. poly-d-lysine, polystyrene, and preculture: p < 0.001. Poly-d-lysine vs. polystyrene, and preculture: p < 0.001. Polystyrene vs. preculture: p < 0.001. An experiment representative of 2 independent experiments is shown. (B) ANOVA analysis: p < 0.0001. Tukey-Kramer Multiple Comparisons post-test: Fibronectin vs. collagen, Matrigel, poly-d-lysine, polystyrene, and preculture: p < 0.001; vs. gelatin p < 0.01. Collagen vs. gelatin, Matrigel, poly-d-lysine, polystyrene, and preculture: p < 0.001. Gelatin vs. Matrigel, poly-d-lysine, and preculture: p < 0.001; vs. polystyrene: p < 0.05. Matrigel vs. polystyrene, poly-d-lysine, and preculture: p < 0.001. Poly-d-lysine vs. polystyrene: p < 0.001; vs. preculture: NS. Polystyrene vs. preculture: p < 0.001. An experiment representative of 2 independent experiments is shown. An experiment representative of 2 independent experiments is shown. (C) Qualitative flow cytometry analysis of CD80 in mDCs recovered from 3-week cultures before and after treatment with inflammatory factors. Grey histograms represent isotype controls. Numbers represent mean fluorescence intensity values. An experiment representative of 2 independent experiments is shown. (D). CFSE dilution analysis. Proliferation of CFSE-stained allogeneic BALB/c lymphocytes was determined by qualitative flow cytometry analysis after 5 day co-culture with the same mDCs as in (C). An experiment representative of 2 independent experiments is shown. Numbers represent percent of CFSE negative cells.