Figure 6
One-week mDC cultures response to inflammatory stimuli. IL-6 (A), IL-1α (B) and nitrites (C) were detected by ELISA analysis on mDC-cultures after 1 week culture in the presence or absence of 100 ng/ml LPS and 20 ng/ml TNFα. An experiment representative of two independent experiments with n = 2 for each condition is shown. (A) IL-6 production by DC cultures. All inflammatory stimuli-treated samples showed significantly higher levels of nitrites when compared to their untreated counterparts as determined by ANOVA analysis followed by Tukey-Kramer Multiple Comparisons post-test. *p < 0.001. (B) IL-1α production by DC cultures. All inflammatory stimuli-treated samples showed significantly higher levels of nitrites when compared to their untreated counterparts as determined by ANOVA analysis followed by Tukey-Kramer Multiple Comparisons post-test. *p < 0.001. (C) Nitrite detection by the Griess method. All inflammatory stimuli-treated samples showed significantly higher levels of nitrites when compared to their untreated counterparts as determined by ANOVA analysis followed by Tukey-Kramer Multiple Comparisons post-test. *p < 0.001.