Figure 3
From: Multidrug-resistance proteins are weak tumor associated antigens for colorectal carcinoma
Analysis of MDP-specific CTL activity against peptide-sensitized T2 target cells. Cytotoxic activity of MDP-specific CTL after at least 3 rounds of restimulation with the indicated MDP mixes was analyzed in standard 51Cr-release assays. MDP-specific reactivity of T cell bulk cultures (Mix-1Tc to Mix-6Tc) was tested against T2 target cells loaded with either the indicated peptide mixes or the given individual MDP. Effector T cells were added at different effector to target cell (E:T) ratios. A representative experiment with CTL derived from donor 2 is shown. The analysis was performed in triplicates with 1.000 effector cells per well and after a 6 h incubation period.