Effect of Cl
MDP-liposome treatment on the survival of MΦ in mice. (A) Cl2MDP-liposome-mediated depletion of monocytes/MΦ from the peripheral blood of mice. Each value represents the mean proportion of monocytes/MΦ ± SD (n = 6). (B) The survival of TAMs in tumor-bearing mice was examined by immunostaining with the rat monoclonal anti-MΦ antibody [RM0029-11H3] and antibodies to F4/80 and CD68 after treatment with Cl2MDP-liposomes. (C) Infiltrating TAMs were counted at × 400 magnification (a 0.17 mm2 field). Data represent the mean MΦ number per field ± SD (10 fields per tumor, 5 tumors per group). *p < 0.05, **p < 0.01, ***p < 0.001 (unpaired Student's t-test).